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Polyclonal Antibody to DC-SIGN (CD209) |
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Cat.No
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Description
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Format
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Unit
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Price(USD)
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MSDS
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| Catalog No: |
IMG-6806A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein A Chromatography |
| Species: |
Ape, Human, Mouse, Orangutan |
| Predicted React: |
Chimpanzee, Macaque, Monkey, Rat, Rhesus Monkey, White-cheeked gibbon |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 2-4 ug/ml |
| Storage: |
Store DC-SIGN (CD209) antibody at 4°C, stable for 6 months. For long-term storage, aliquot and store antibody at -20°C. |
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Background
DC-SIGN, also called CD209, is a C type lectin receptor and an acronym for Dendritic Cell-Specific intracellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin protein. DC-SIGN (CD209) is a 45kDa receptor which binds mannans and glycans on bacteria, viruses and fungi (1). Expressed on Dendritic Cells (DCs) and DC-like macrophages, DC-SIGN is an important cell interaction molecule mediating DC-T cell or DC-Neutrophil responses. DC-SIGN is especially important in DC Th1 interaction, regulating Th1 activity through it‘s role as a pathogen receptor (2-4). Interaction between DC-SIGN and TLR4 has been demonstrated for induction of inflammatory responses (5). As a CLR and innate immune receptor, DC-SIGN detection and quantitation with antibody probes are used as both a phenotype and indicator of a functional role in anti-microbial responses of DC’s. |
Antigen
A portion of amino acids 320-370 from human DC-SIGN (CD209) was used as the immunogen for this antibody. |
Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, ape, and orangutan, 94% in old World monkey, rhesus monkey, macaque, white-cheeked gibbon, and chimpanzee, 89% in rat, and 83% homologous in mouse. |
Genebank Info (Protein)
NP_066978.1 |
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Western blot analysis of DC-SIGN (CD209) in human ThP-1 lysate in the A) absence and B) presence of immunizing peptide and C) mouse RAW lysate using DC-SIGN antibody at 3 ug/ml. |
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Reference
1. www.functionalglycomics.org/CFGparadigms/index.php/DC-SIGN
2. Geijtenbeek TB, et al 2000. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 100:575-585
3. van Gisbergen KP, et al 2005 Neutrophils mediate immune modulation of dendritic cells through glycosylation-dependent interactions between MAC-1 and DC-SIGN. J Exp Med. 201:1281-1292.
4. Steeghs L, et al 2006. Neisseria meningitidis expressing Lgtb lipopolysaccharide targets DC-SIGN and modulates dendritic cell function. Cell Microbiol. 8:316-325
5. van Stijn CM, et al 2010. Schistosoma mansoni worm glycolipids induce an inflammatory phenotype in human dendritic cells by cooperation of TLR4 and DC-SIGN. Mol Immunol. 47:1544-1552
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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